Flow cytometry and FISH to measure the average length of telomeres (flow FISH)
Title
Flow cytometry and FISH to measure the average length of telomeres (flow FISH)
Creator
Baerlocher GM; Vulto I; de Jong G; Lansdorp PM
Identifier
Publisher
Nature Protocols
Date
2006
Subject
Telemeres
Description
Telomeres have emerged as crucial cellular elements in aging and various diseases including cancer. To measure the average length of telomere repeats in cells, we describe our protocols that use fluorescent in situ hybridization (FISH) with labeled peptide nucleic acid (PNA) probes specific for telomere repeats in combination with fluorescence measurements by flow cytometry (flow FISH). Flow FISH analysis can be performed using commercially available flow cytometers, and has the unique advantage over other methods for measuring telomere length of providing multi-parameter information on the length of telomere repeats in thousands of individual cells. The accuracy and reproducibility of the measurements is augmented by the automation of most pipetting (aspiration and dispensing) steps, and by including an internal standard (control cells) with a known telomere length in every tube. The basic protocol for the analysis of nucleated blood cells from 22 different individuals takes about 12 h spread over 2-3 days.
2006
Rights
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Type
Journal Article
Citation List Month
Backlog
URL Address
Citation
Baerlocher GM; Vulto I; de Jong G; Lansdorp PM, “Flow cytometry and FISH to measure the average length of telomeres (flow FISH),” Pediatric Palliative Care Library, accessed May 2, 2024, https://pedpalascnetlibrary.omeka.net/items/show/13632.