Lymphocytes upregulate signal sequence-encoding proopiomelanocortin mRNA and beta-endorphin during painful inflammation in vivo

Title

Lymphocytes upregulate signal sequence-encoding proopiomelanocortin mRNA and beta-endorphin during painful inflammation in vivo

Creator

Sitte N; Busch M; Mousa SA; Labuz D; Rittner H; Gore C; Krause H; Stein C; Schafer M

Publisher

Journal Of Neuroimmunology

Date

2007

Subject

Male; Time Factors; Animals; Rats; Biomarkers of Pain; RNA; beta-Endorphin/metabolism; Freund's Adjuvant; Wistar; Lymphocytes/metabolism; Flow Cytometry/methods; Gene Expression Regulation/drug effects/physiology; Inflammation/chemically induced/complications/pathology; Messenger/metabolism; Pain/etiology/pathology; Pro-Opiomelanocortin/genetics/metabolism; Protein Sorting Signals; Reverse Transcriptase Polymerase Chain Reaction/methods

Description

Proopiomelanocortin (POMC)-derived beta-endorphin1-31 (END) released from immune cells inhibits inflammatory pain. We examined the expression of END and POMC mRNA encoding the signal sequence required for entry of the nascent polypeptide into the regulated secretory pathway in lymphocytes of rats with inflamed hindpaws. Within 12 h of inflammation, END increased in popliteal lymph nodes and at 96 h the intraplantar neutralization of END exacerbated pain. Lymphocytes expressed POMC, END, and full-length POMC mRNA. Semi-nested PCR revealed 8-fold increased exon 2-3 spanning POMC mRNA. Thus, painful inflammation enhances signal sequence-encoding lymphocytic POMC mRNA needed for regulated secretion of functionally active END.
2007

Rights

Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).

Type

Journal Article

Citation List Month

Backlog

Pages

133-145

Issue

1

Volume

183

Citation

Sitte N; Busch M; Mousa SA; Labuz D; Rittner H; Gore C; Krause H; Stein C; Schafer M, “Lymphocytes upregulate signal sequence-encoding proopiomelanocortin mRNA and beta-endorphin during painful inflammation in vivo,” Pediatric Palliative Care Library, accessed May 22, 2022, https://pedpalascnetlibrary.omeka.net/items/show/14384.

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