Incubation time required for neonatal blood cultures to become positive

Title

Incubation time required for neonatal blood cultures to become positive

Creator

Jardine L; Davies MW; Faoagali J

Publisher

Journal Of Paediatrics And Child Health

Date

2006

Subject

Humans; infant; Time Factors; Birth Weight; Newborn; retrospective studies; Bacteremia/blood/diagnosis/microbiology; Bacteriological Techniques/methods; Blood/microbiology; Sepsis/blood/diagnosis/microbiology

Description

AIM: We aimed to determine the laboratory detection time of bacteraemia in neonatal blood cultures, and whether this differed by: organism; samples deemed to represent true bacteraemia versus contaminants; and blood cultures collected from an infant or=48 h of age (late). METHODS: A retrospective audit of all positive blood cultures collected from neonates in the Grantley Stable Neonatal Unit, Royal Women's Hospital, Brisbane, between 1 January 2000 and 31 December 2004 was undertaken. The bacteraemia detection method used was the BacTAlert system with Peds bottles. RESULTS: Two hundred and three positive blood cultures were included in the analysis. One hundred and sixteen (57%) were deemed septicaemia, 87 (43%) were deemed contaminants. The median (interquartile range) time to positivity for positive blood cultures deemed septicaemia and contaminants were 15.9 (11.6, 22.2) and 30.2 (20.4, 43.9) h, respectively. Fifty-six (28%) positive blood cultures were collected when infants were or=48 h of age. Post hoc analysis revealed that the time to positivity for early septicaemia was 13.7 (11, 16.7) h; early contaminant was 25.2 (19.2, 33.8) h; late septicaemia was 17.2 (12.2, 23.4) h; and late contaminant was 37.9 (21.7, 51.2) h. The time to positivity for: Group B streptococcus was 9.3 (8.2, 11.0) h; Escherichia coli was 11.3 (10.0, 13.5) h; and coagulase-negative staphylococci was 28.9 (20.5, 41.2) h. CONCLUSION: The incubation time for positive blood cultures significantly differs by organism type and whether they are considered early or late septicaemia versus contaminants. We recommend that: infants who are or=48 h of age at the time of collection should continue antibiotic treatment for at least 48 h before cessation is considered.
2006

Rights

Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).

Type

Journal Article

Citation List Month

Backlog

Pages

797-802

Issue

12

Volume

42

Citation

Jardine L; Davies MW; Faoagali J, “Incubation time required for neonatal blood cultures to become positive,” Pediatric Palliative Care Library, accessed November 28, 2021, https://pedpalascnetlibrary.omeka.net/items/show/13461.

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