Human neutrophils as a source of nociceptin: a novel link between pain and inflammation

Title

Human neutrophils as a source of nociceptin: a novel link between pain and inflammation

Creator

Fiset ME; Gilbert C; Poubelle PE; Pouliot M

Identifier

Publisher

Biochemistry

Date

2003

Subject

Humans; Molecular Sequence Data; Biomarkers of Pain; Receptors; Neutrophils/metabolism; Arthritis/metabolism; Cyclic AMP/metabolism; Inflammation/metabolism; Opioid Peptides/metabolism; Opioid/agonists/genetics/metabolism; Pain/metabolism; Phosphorylation; Protein-Tyrosine Kinases/metabolism; Proto-Oncogene Proteins c-hck; Proto-Oncogene Proteins/metabolism; Synovial Fluid/chemistry/immunology

Description

Nociceptin is a neuropeptide sharing sequence homology with classical opioid peptides but with a distinct pharmacological profile. Through activation of its receptor, NociR, nociceptin has been linked with several physiological functions in the central nervous system including memory, locomotion, and processing of pain signals. Recently, peripheral blood neutrophils (PMNs) were demonstrated to express a functional NociR, a result suggesting that additional functions of the neuropeptide remain to be elucidated. The present study investigated the possibility that PMNs may be a source of nociceptin and whether the neuropeptide elicits PMN early responses. We observed the presence of nociceptin in the synovial fluids from arthritic patients, an inflammatory milieu typically containing high numbers of PMNs. In addition, freshly isolated PMNs were found to express and secrete nociceptin following degranulation, identifying these inflammatory cells as a novel source of the neuropeptide. Incubation of PMNs with nociceptin elicited a specific pattern of cellular protein phosphorylation on tyrosine residues in a rapid and transient fashion. Moreover, nociceptin prevented intracellular accumulation of cAMP in fMLP-stimulated PMNs, an effect mimicked by the specific NociR synthetic agonist, Ro 64-6198. Taken together, these results show that nociceptin/NociR is present and functional in human neutrophils, and the results identify a novel dialogue pathway between neural and immune tissues.
2003

Rights

Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).

Type

Journal Article

Citation List Month

Backlog

Pages

10498-10505

Issue

35

Volume

42

Citation

Fiset ME; Gilbert C; Poubelle PE; Pouliot M, “Human neutrophils as a source of nociceptin: a novel link between pain and inflammation,” Pediatric Palliative Care Library, accessed October 25, 2021, https://pedpalascnetlibrary.omeka.net/items/show/12980.

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