Age-related changes in mitogen-induced beta-endorphin release from human peripheral blood mononuclear cells

Title

Age-related changes in mitogen-induced beta-endorphin release from human peripheral blood mononuclear cells

Creator

Manfredi B; Clementi E; Sacerdote P; Bassetti M; Panerai AE

Publisher

Peptides

Date

1995

Subject

Humans; Adult; Middle Aged; Biomarkers of Pain; Leukocytes; Aging/blood; beta-Endorphin/metabolism; Calcium-Transporting ATPases/antagonists & inhibitors; Calcium/blood; Concanavalin A/pharmacology; Homeostasis/drug effects; Ionomycin/pharmacology; Mitogens/pharmacology; Mononuclear/cytology/drug effects; Phytohemagglutinins/pharmacology; Terpenes/pharmacology; Thapsigargin

Description

beta-Endorphin is an opioid peptide synthesized in the pituitary, hypothalamus, and immunocytes, known to affect immune responses both when added in vitro and when its synthesis is increased in vivo (e.g., during stress). We show here that, similar to its concentrations in peripheral blood mononuclear cells, the release of the opioid peptide from these cells after stimulation with polyclonal mitogens such as PHA or Con-A is also age dependent. Moreover, the effect of both mitogens on Ca2+ homeostasis changes with age. Finally, the ionophore ionomycin and the Ca2+ ATPase blocker thapsigargin induce the same age related effect on beta-endorphin release. For these reasons, we suggest that calcium homeostasis might be important for the differences observed in the release of the opioid from cells obtained from younger ( or = 45 years) volunteers.
1995

Rights

Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).

Type

Journal Article

Citation List Month

Backlog

Pages

699-706

Issue

4

Volume

16

Citation

Manfredi B; Clementi E; Sacerdote P; Bassetti M; Panerai AE, “Age-related changes in mitogen-induced beta-endorphin release from human peripheral blood mononuclear cells,” Pediatric Palliative Care Library, accessed September 24, 2021, https://pedpalascnetlibrary.omeka.net/items/show/12297.

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