1
40
10
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Text
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<a href="http://doi.org/10.1002/ajmg.a.31841" target="_blank" rel="noreferrer">http://doi.org/10.1002/ajmg.a.31841</a>
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Title
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Progressive cerebral vascular degeneration with mitochondrial encephalopathy
Publisher
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American Journal Of Medical Genetics.Part A
Date
A point or period of time associated with an event in the lifecycle of the resource
2008
Subject
The topic of the resource
Child; Female; Humans; Cerebrovascular Circulation; Q3 Literature Search; Acyl-CoA Dehydrogenase/deficiency/genetics; Brain/blood supply/physiopathology; Leu/genetics; Magnetic Resonance Angiography; MELAS Syndrome/ge [Genetics]; Point Mutation; RNA; Transfer
Creator
An entity primarily responsible for making the resource
Longo N; Schrijver I; Vogel H; Pique LM; Cowan TM; Pasquali M; Steinberg GK; Hedlund GL; Ernst SL; Gallagher RC; Enns GM
Description
An account of the resource
MELAS (mitochondrial encephalopathy with lactic acidosis and stroke-like episodes) is a maternally inherited disorder characterized by recurrent cerebral infarctions that do not conform to discreet vascular territories. Here we report on a patient who presented at 7 years of age with loss of consciousness and severe metabolic acidosis following vomiting and dehydration. She developed progressive sensorineural hearing loss, myopathy, ptosis, short stature, and mild developmental delays after normal early development. Biochemical testing identified metabolites characteristic of medium-chain acyl-CoA dehydrogenase (MCAD) deficiency (hexanoylglycine and suberylglycine), but also severe lactic acidemia (10-25 mM) and, in urine, excess of lactic acid, intermediates of the citric cycle, and marked ketonuria, suggesting mitochondrial dysfunction. She progressed rapidly to develop temporary cortical blindness. Brain imaging indicated generalized atrophy, more marked on the left side, in addition to white matter alterations consistent with a mitochondrial disorder. Magnetic resonance angiography indicated occlusion of the left cerebral artery with development of collateral circulation (Moyamoya syndrome). This process worsened over time to involve the other side of the brain. A muscle biopsy indicated the presence of numerous ragged red fibers. Molecular testing confirmed compound heterozygosity for the common mutation in the MCAD gene (985A>G) and a second pathogenic mutation (233T>C). MtDNA testing indicated that the muscle was almost homoplasmic for the 3243A>T mutation in tRNALeu, with a lower mutant load (about 50% heteroplasmy) in blood and skin fibroblasts. These results indicate that mitochondrial disorders may be associated with severe vascular disease resulting in Moyamoya syndrome. The contribution of the concomitant MCAD deficiency to the development of the phenotype in this case is unclear.
2008
Identifier
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<a href="http://doi.org/10.1002/ajmg.a.31841" target="_blank" rel="noreferrer">10.1002/ajmg.a.31841</a>
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Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
2008
Acyl-CoA Dehydrogenase/deficiency/genetics
American Journal Of Medical Genetics.Part A
Backlog
Brain/blood supply/physiopathology
Cerebrovascular Circulation
Child
Cowan TM
Enns GM
Ernst SL
Female
Gallagher RC
Hedlund GL
Humans
Journal Article
Leu/genetics
Longo N
Magnetic Resonance Angiography
MELAS Syndrome/ge [Genetics]
Pasquali M
Pique LM
Point Mutation
Q3 Scoping Review Results
RNA
Schrijver I
Steinberg GK
Transfer
Vogel H
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1016/j.jneuroim.2006.11.033" target="_blank" rel="noreferrer">http://doi.org/10.1016/j.jneuroim.2006.11.033</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Lymphocytes upregulate signal sequence-encoding proopiomelanocortin mRNA and beta-endorphin during painful inflammation in vivo
Publisher
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Journal Of Neuroimmunology
Date
A point or period of time associated with an event in the lifecycle of the resource
2007
Subject
The topic of the resource
Male; Time Factors; Animals; Rats; Biomarkers of Pain; RNA; beta-Endorphin/metabolism; Freund's Adjuvant; Wistar; Lymphocytes/metabolism; Flow Cytometry/methods; Gene Expression Regulation/drug effects/physiology; Inflammation/chemically induced/complications/pathology; Messenger/metabolism; Pain/etiology/pathology; Pro-Opiomelanocortin/genetics/metabolism; Protein Sorting Signals; Reverse Transcriptase Polymerase Chain Reaction/methods
Creator
An entity primarily responsible for making the resource
Sitte N; Busch M; Mousa SA; Labuz D; Rittner H; Gore C; Krause H; Stein C; Schafer M
Description
An account of the resource
Proopiomelanocortin (POMC)-derived beta-endorphin1-31 (END) released from immune cells inhibits inflammatory pain. We examined the expression of END and POMC mRNA encoding the signal sequence required for entry of the nascent polypeptide into the regulated secretory pathway in lymphocytes of rats with inflamed hindpaws. Within 12 h of inflammation, END increased in popliteal lymph nodes and at 96 h the intraplantar neutralization of END exacerbated pain. Lymphocytes expressed POMC, END, and full-length POMC mRNA. Semi-nested PCR revealed 8-fold increased exon 2-3 spanning POMC mRNA. Thus, painful inflammation enhances signal sequence-encoding lymphocytic POMC mRNA needed for regulated secretion of functionally active END.
2007
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.jneuroim.2006.11.033" target="_blank" rel="noreferrer">10.1016/j.jneuroim.2006.11.033</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
2007
Animals
Backlog
beta-Endorphin/metabolism
Biomarkers of Pain
Busch M
Flow Cytometry/methods
Freund's Adjuvant
Gene Expression Regulation/drug effects/physiology
Gore C
Inflammation/chemically induced/complications/pathology
Journal Article
Journal Of Neuroimmunology
Krause H
Labuz D
Lymphocytes/metabolism
Male
Messenger/metabolism
Mousa SA
Pain/etiology/pathology
Pro-Opiomelanocortin/genetics/metabolism
Protein Sorting Signals
Rats
Reverse Transcriptase Polymerase Chain Reaction/methods
Rittner H
RNA
Schafer M
Sitte N
Stein C
Time Factors
Wistar
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1016/s0024-3205(02)02415-3" target="_blank" rel="noreferrer">http://doi.org/10.1016/s0024-3205(02)02415-3</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Cytokine and hormone profiles in mice subjected to handling combined with rectal temperature measurement stress and handling only stress
Publisher
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Life Sciences
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
Subject
The topic of the resource
Female; Animals; Mice; Stress; beta-Endorphin/blood; Biomarkers of Pain; RNA; Killer Cells; Body Temperature/physiology; Adrenocorticotropic Hormone/blood; Cytokines/blood/metabolism; Handling (Psychology); Hormones/blood/metabolism; Inbred BALB C; Messenger/biosynthesis; Natural/physiology; Psychological/metabolism; Spleen/cytology/metabolism
Creator
An entity primarily responsible for making the resource
Hale KD; Weigent DA; Gauthier DK; Hiramoto RN; Ghanta VK
Description
An account of the resource
Stress is known to either up or down regulate immunity. In this study, mice were subjected to handling combined with rectal temperature measurement (RTM) stress or handling only stress. We investigated whether there were any significant differences in the effect of handling combined with RTM and handling only on NK cell activity, serum cytokine (IL-1beta, IL-6, and TNF-alpha) and ACTH and beta-endorphin levels, and splenic cytokine (IL-1beta, IL-6, TNF-alpha, IFN-alpha, and IFN-beta) levels. Circulating cytokines and hormones and splenic cytokine mRNA levels were measured in individual mice. NK cell activity was significantly increased in both stress groups when compared to the control group. Handling combined with RTM produced significantly increased serum levels of IL-1beta, IL-6, and beta-endorphin. Serum IL-1beta, ACTH, and beta-endorphin were elevated significantly in the handling only group. Splenic TNFalpha mRNA in both of the stress groups and IL-6 mRNA in handling only group decreased significantly. Our observations are supported by existing literature demonstrating that various stressors have differential effects on immune functions and the neuroendocrine hormones and cytokines, which regulate them.
2003
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0024-3205(02)02415-3" target="_blank" rel="noreferrer">10.1016/s0024-3205(02)02415-3</a>
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Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
2003
Adrenocorticotropic Hormone/blood
Animals
Backlog
beta-Endorphin/blood
Biomarkers of Pain
Body Temperature/physiology
Cytokines/blood/metabolism
Female
Gauthier DK
Ghanta VK
Hale KD
Handling (Psychology)
Hiramoto RN
Hormones/blood/metabolism
Inbred BALB C
Journal Article
Killer Cells
Life Sciences
Messenger/biosynthesis
Mice
Natural/physiology
Psychological/metabolism
RNA
Spleen/cytology/metabolism
Stress
Weigent DA
-
Text
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Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1097/00000542-200401000-00024" target="_blank" rel="noreferrer">http://doi.org/10.1097/00000542-200401000-00024</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Mobilization of opioid-containing polymorphonuclear cells by hematopoietic growth factors and influence on inflammatory pain
Publisher
An entity responsible for making the resource available
Anesthesiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
Subject
The topic of the resource
Male; Animals; Rats; Biomarkers of Pain; Inflammation/complications; RNA; Pain Measurement/drug effects; Radioimmunoassay; Pain/drug therapy/physiopathology; Wistar; Neutrophils/metabolism; Messenger/biosynthesis; DNA Primers; Reverse Transcriptase Polymerase Chain Reaction; Flow Cytometry; Cell Adhesion Molecules/pharmacology; Cell Separation; Chemokines/biosynthesis; Chemotaxis; Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology; Hindlimb/physiology; Leukocyte/drug effects; Light; Narcotics/metabolism/pharmacology; Stem Cell Factor/pharmacology
Creator
An entity primarily responsible for making the resource
Brack A; Rittner HL; Machelska H; Beschmann K; Sitte N; Schafer M; Stein C
Description
An account of the resource
BACKGROUND: Leukocytes can control inflammatory pain by secretion of opioid peptides, stimulated by cold-water swimming or local injection of corticotropin-releasing factor, and subsequent activation of opioid receptors on peripheral sensory neurons. This study investigated whether mobilization of polymorphonuclear cells (PMN) by granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) enhances immigration of opioid-containing PMN and peripheral opioid analgesia in rats with Freund complete adjuvant-induced hind paw inflammation. METHODS: In circulating PMN of rats treated with G-CSF+SCF and sham-treated rats, opioid peptide content was measured by radioimmunoassay. Expression of adhesion molecules (CD62L, CD49d, CD18), in vitro migration in the Boyden chamber, and infiltrating leukocytes were analyzed by flow cytometry. Chemokine messenger RNA transcription was quantified by LightCycler polymerase chain reaction. Paw pressure threshold was measured at baseline, after cold-water swimming, and after injection of corticotropin-releasing factor. RESULTS: G-CSF+SCF treatment increased circulating PMN (11-fold, P 0.05). CONCLUSIONS: G-CSF+SCF mobilized circulating opioid-containing PMN but had a minor influence on cell migration and peripheral analgesia, probably because of the low expression of chemokines in the inflamed paw and one of the decreased beta-endorphin content in PMN.
2004
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1097/00000542-200401000-00024" target="_blank" rel="noreferrer">10.1097/00000542-200401000-00024</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
2004
Anesthesiology
Animals
Backlog
Beschmann K
Biomarkers of Pain
Brack A
Cell Adhesion Molecules/pharmacology
Cell Separation
Chemokines/biosynthesis
Chemotaxis
DNA Primers
Flow Cytometry
Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
Hindlimb/physiology
Inflammation/complications
Journal Article
Leukocyte/drug effects
Light
Machelska H
Male
Messenger/biosynthesis
Narcotics/metabolism/pharmacology
Neutrophils/metabolism
Pain Measurement/drug effects
Pain/drug therapy/physiopathology
Radioimmunoassay
Rats
Reverse Transcriptase Polymerase Chain Reaction
Rittner HL
RNA
Schafer M
Sitte N
Stein C
Stem Cell Factor/pharmacology
Wistar
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1097/01.ALC.0000095636.44770.55" target="_blank" rel="noreferrer">http://doi.org/10.1097/01.ALC.0000095636.44770.55</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Decreased proopiomelanocortin mRNA in lymphocytes of chronic alcoholics after intravenous human corticotropin releasing factor injection
Publisher
An entity responsible for making the resource available
Alcoholism, Clinical And Experimental Research
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
Subject
The topic of the resource
Female; Humans; Male; Adult; Aged; Middle Aged; Biomarkers of Pain; Nonparametric; Statistics; RNA; Corticotropin-Releasing Hormone/pharmacology; Alcoholism/blood/metabolism; Lymphocytes/drug effects/metabolism; Messenger/biosynthesis/genetics; Pro-Opiomelanocortin/biosynthesis/genetics
Creator
An entity primarily responsible for making the resource
Rosenberger P; Muhlbauer E; Weissmuller T; Rommelspacher H; Sinha P; Wernecke KD; Finckh U; Rettig J; Kox WJ; Spies CD
Description
An account of the resource
BACKGROUND: Alcohol abuse may involve an altered neuroendocrine response that mediates lymphocyte-derived proopiomelanocortin (POMC) production and inflammation. We investigated POMC messenger RNA (mRNA) expression in human lymphocytes ex vivo and their relation to plasma ACTH and immunoreactive beta-endorphin (IR-beta-EP) after intravenous injection of human corticotropin releasing factor (hCRF) in chronic alcoholics (n = 12) and nonalcoholics (n = 12) before surgery. Lipopolysaccharide-stimulated interleukin (IL)-1 receptor antagonist (IL-1 Ra) as a marker for chronic inflammation was determined. METHODS: Chronic alcohol abuse was diagnosed according to DSM-IV criteria and alcohol consumption >60 g/day. A reverse transcription-polymerase chain reaction method with total RNA and subsequent solid phase minisequencing was used to quantify lymphocytic POMC mRNA after intravenous hCRF injection. Plasma ACTH, cortisol, and lipopolysaccharide-stimulated IL-1 Ra of monocytes were measured by enzyme-linked immunosorbent assay, and plasma IR-beta-EP was measured by using radioimmunoassay. RESULTS: Baseline values of POMC mRNA content in lymphocytes and IL-1 Ra of chronic alcoholics were significantly increased compared with nonalcoholics (p < 0.01). Thirty minutes after intravenous hCRF injection, a significant increase of lymphocytic POMC mRNA was measured (p < 0.05) in nonalcoholics, whereas in chronic alcoholics a significant decrease was observed (p < 0.05). CONCLUSIONS: Chronic alcoholic patients had an altered neuroendocrine immune axis before intravenous hCRF administration and were not able to adjust to intravenous CRF injection by increasing lymphocytic POMC mRNA expression.
2003
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1097/01.ALC.0000095636.44770.55" target="_blank" rel="noreferrer">10.1097/01.ALC.0000095636.44770.55</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
2003
Adult
Aged
Alcoholism, Clinical And Experimental Research
Alcoholism/blood/metabolism
Backlog
Biomarkers of Pain
Corticotropin-Releasing Hormone/pharmacology
Female
Finckh U
Humans
Journal Article
Kox WJ
Lymphocytes/drug effects/metabolism
Male
Messenger/biosynthesis/genetics
Middle Aged
Muhlbauer E
Nonparametric
Pro-Opiomelanocortin/biosynthesis/genetics
Rettig J
RNA
Rommelspacher H
Rosenberger P
Sinha P
Spies CD
Statistics
Weissmuller T
Wernecke KD
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1016/0306-4522(92)90509-z" target="_blank" rel="noreferrer">http://doi.org/10.1016/0306-4522(92)90509-z</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Gene expression and localization of opioid peptides in immune cells of inflamed tissue: functional role in antinociception
Publisher
An entity responsible for making the resource available
Neuroscience
Date
A point or period of time associated with an event in the lifecycle of the resource
1992
Subject
The topic of the resource
Male; Pain Measurement; Analysis of Variance; Animals; Nucleic Acid Hybridization; Rats; Non-U.S. Gov't; Research Support; Biomarkers of Pain; RNA; Biomarkers Reference List; Inbred Strains; beta-Endorphin/genetics/metabolism; Calcitonin Gene-Related Peptide/analysis/metabolism; Endorphins/analysis/genetics/metabolism; Freund's Adjuvant; Gene Expression/radiation effects; Hindlimb; Inflammation/immunology/physiopathology; Messenger/genetics/metabolism; Nerve Fibers/physiology/ultrastructure; Oligonucleotide Probes; Pain/immunology/physiopathology; T-Lymphocytes/immunology/pathology; Whole-Body Irradiation
Creator
An entity primarily responsible for making the resource
Przewlocki R; Hassan AH; Lason W; Epplen C; Herz A; Stein C
Description
An account of the resource
Our previous studies indicate that endogenous opioids (primarily beta-endorphin) released during stressful stimuli can interact with peripheral opioid receptors to inhibit nociception in inflamed tissue of rats. This study sought to localize opioid precursor mRNAs and opioid peptides deriving therefrom in inflamed tissue, identify opioid containing cells and demonstrate their functional significance in the inhibition of nociception. In rats with Freund's adjuvant-induced unilateral hindpaw inflammation we show that: (i) pro-opiomelanocortin and proenkephalin-mRNAs (but not prodynorphin mRNA) are abundant in cells of inflamed, but absent in non-inflamed tissue; (ii) numerous cells infiltrating the inflamed subcutaneous tissue are stained intensely with beta-endorphin and [Met]enkephalin (but only few scattered cells with dynorphin) antibodies; (iii) beta-endorphin is present in T- and B-lymphocytes, monocytes and macrophages; and (iv) whole-body irradiation suppresses stress-induced antinociception in the inflamed paw. Taken together, these data suggest that endogenous opioid peptides are synthesized and processed within various types of immune cells at the site of inflammation. Immunosuppression abolishes the intrinsic antinociception in inflammatory tissue confirming the functional significance of these cells.
1992
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0306-4522(92)90509-z" target="_blank" rel="noreferrer">10.1016/0306-4522(92)90509-z</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
1992
Analysis of Variance
Animals
Backlog
beta-Endorphin/genetics/metabolism
Biomarkers of Pain
Biomarkers Reference List
Calcitonin Gene-Related Peptide/analysis/metabolism
Endorphins/analysis/genetics/metabolism
Epplen C
Freund's Adjuvant
Gene Expression/radiation effects
Hassan AH
Herz A
Hindlimb
Inbred Strains
Inflammation/immunology/physiopathology
Journal Article
Lason W
Male
Messenger/genetics/metabolism
Nerve Fibers/physiology/ultrastructure
Neuroscience
Non-U.S. Gov't
Nucleic Acid Hybridization
Oligonucleotide Probes
Pain Measurement
Pain/immunology/physiopathology
Przewlocki R
Rats
Research Support
RNA
Stein C
T-Lymphocytes/immunology/pathology
Whole-Body Irradiation
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1038/nm0897-831" target="_blank" rel="noreferrer">http://doi.org/10.1038/nm0897-831</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Pain killers of the immune system
Publisher
An entity responsible for making the resource available
Nature Medicine
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
Subject
The topic of the resource
Animals; Rats; Biomarkers of Pain; RNA; Immunohistochemistry; Radioimmunoassay; beta-Endorphin/biosynthesis/physiology; Inflammation/pathology/physiopathology; Messenger/genetics; Pain/pathology/physiopathology; Pro-Opiomelanocortin/genetics; T-Lymphocytes/metabolism
Creator
An entity primarily responsible for making the resource
Sharp B; Yaksh T
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1038/nm0897-831" target="_blank" rel="noreferrer">10.1038/nm0897-831</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
Description
An account of the resource
1997
1997
Animals
Backlog
beta-Endorphin/biosynthesis/physiology
Biomarkers of Pain
Immunohistochemistry
Inflammation/pathology/physiopathology
Journal Article
Messenger/genetics
Nature Medicine
Pain/pathology/physiopathology
Pro-Opiomelanocortin/genetics
Radioimmunoassay
Rats
RNA
Sharp B
T-Lymphocytes/metabolism
Yaksh T
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1172/jci119506" target="_blank" rel="noreferrer">http://doi.org/10.1172/jci119506</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Immune cell-derived beta-endorphin. Production, release, and control of inflammatory pain in rats
Publisher
An entity responsible for making the resource available
The Journal Of Clinical Investigation
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
Subject
The topic of the resource
Humans; Male; Time Factors; Analysis of Variance; Animals; Regression Analysis; Rats; Biomarkers of Pain; RNA; Genetic; Biomarkers Reference List; Inflammation/physiopathology; Freund's Adjuvant; Hindlimb; Pain/immunology/physiopathology; Corticotropin-Releasing Hormone/pharmacology; Wistar; Messenger/biosynthesis; beta-Endorphin/biosynthesis; Interleukin-1/pharmacology; Lymph Nodes/metabolism; Pro-Opiomelanocortin/biosynthesis; T-Lymphocytes/drug effects/immunology/metabolism; Transcription
Creator
An entity primarily responsible for making the resource
Cabot PJ; Carter L; Gaiddon C; Zhang Q; Schafer M; Loeffler JP; Stein C
Description
An account of the resource
Localized inflammation of a rat's hindpaw elicits an accumulation of beta-endorphin-(END) containing immune cells. We investigated the production, release, and antinociceptive effects of lymphocyte-derived END in relation to cell trafficking. In normal animals, END and proopiomelanocortin mRNA were less abundant in circulating lymphocytes than in those residing in lymph nodes (LN), suggesting that a finite cell population produces END and homes to LN. Inflammation increased proopiomelanocortin mRNA in cells from noninflamed and inflamed LN. However, END content was increased only in inflamed paw tissue and noninflamed LN-immune cells. Accordingly, corticotropin-releasing factor and IL-1beta released significantly more END from noninflamed than from inflamed LN-immune cells. This secretion was receptor specific, calcium dependent, and mimicked by potassium, consistent with vesicular release. Finally, both agents, injected into the inflamed paw, induced analgesia which was blocked by the co-administration of antiserum against END. Together, these findings suggest that END-producing lymphocytes home to inflamed tissue where they secrete END to reduce pain. Afterwards they migrate to the regional LN, depleted of the peptide. Consistent with this notion, immunofluorescence studies of cell suspensions revealed that END is contained predominantly within memory-type T cells. Thus, the immune system is important for the control of inflammatory pain. This has implications for the understanding of pain in immunosuppressed conditions like cancer or AIDS.
1997
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1172/jci119506" target="_blank" rel="noreferrer">10.1172/jci119506</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
1997
Analysis of Variance
Animals
Backlog
beta-Endorphin/biosynthesis
Biomarkers of Pain
Biomarkers Reference List
Cabot PJ
Carter L
Corticotropin-Releasing Hormone/pharmacology
Freund's Adjuvant
Gaiddon C
Genetic
Hindlimb
Humans
Inflammation/physiopathology
Interleukin-1/pharmacology
Journal Article
Loeffler JP
Lymph Nodes/metabolism
Male
Messenger/biosynthesis
Pain/immunology/physiopathology
Pro-Opiomelanocortin/biosynthesis
Rats
Regression Analysis
RNA
Schafer M
Stein C
T-Lymphocytes/drug effects/immunology/metabolism
The Journal Of Clinical Investigation
Time Factors
transcription
Wistar
Zhang Q
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1210/endo.133.5.8404637" target="_blank" rel="noreferrer">http://doi.org/10.1210/endo.133.5.8404637</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
What do we know about the expression of proopiomelanocortin transcripts and related peptides in lymphoid tissue?
Publisher
An entity responsible for making the resource available
Endocrinology
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
Subject
The topic of the resource
Humans; Animals; Biomarkers of Pain; RNA; Messenger/metabolism; Pro-Opiomelanocortin/genetics; Lymphoid Tissue/metabolism
Creator
An entity primarily responsible for making the resource
Sharp B; Linner K
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1210/endo.133.5.8404637" target="_blank" rel="noreferrer">10.1210/endo.133.5.8404637</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
Description
An account of the resource
1993
1993
Animals
Backlog
Biomarkers of Pain
Endocrinology
Humans
Journal Article
Linner K
Lymphoid Tissue/metabolism
Messenger/metabolism
Pro-Opiomelanocortin/genetics
RNA
Sharp B
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1210/endo.133.5.8404638" target="_blank" rel="noreferrer">http://doi.org/10.1210/endo.133.5.8404638</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Analysis of proopiomelanocortin (POMC) messenger ribonucleic acid and POMC-derived peptides in human peripheral blood mononuclear cells: no evidence for a lymphocyte-derived POMC system
Publisher
An entity responsible for making the resource available
Endocrinology
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
Subject
The topic of the resource
Humans; Molecular Sequence Data; Biomarkers of Pain; RNA; Base Sequence; beta-Endorphin/analysis; Biomarkers Reference List; Leukocytes; Lymphocytes/metabolism; Polymerase Chain Reaction; Chromatography; High Pressure Liquid; Pro-Opiomelanocortin/genetics; Mononuclear/metabolism; Blotting; Cathepsin D/metabolism; DNA Probes; Gel; Messenger/blood; Northern; Peptide Fragments/analysis; Peptide Mapping; Southern
Creator
An entity primarily responsible for making the resource
van Woudenberg AD; Metzelaar MJ; van der Kleij AA; de Wied D; Burbach JP; Wiegant VM
Description
An account of the resource
A number of recent studies suggest that cells of the immune system, e.g. peripheral blood mononuclear cells (PBMC), can synthesize and process POMC and secrete POMC-derived peptides, such as ACTH and endorphins, upon immune and hormonal challenges. From this, it has been proposed that POMC-derived peptides originating from lymphoid cells can function as hormones, for instance in a lymphoid-adrenal axis. In view of the important physiological implications of this proposal, the present study was designed to investigate the expression of the POMC gene in human PBMC and the production by these cells of alpha-, beta-, and gamma-endorphins (alpha E, beta E, and gamma E) peptides that are established end products of the posttranslational processing of POMC. PBMC of individual donors were used uncultured (fresh cells) or cultured for 24 and 48 h in the presence and absence of Concanavalin-A (Con-A), bacterial lipopolysaccharide, phytohemagglutinin, or CRH, and vasopressin, conditions that reportedly stimulate POMC activity in those cells, to investigate the presence of POMC transcripts by analysis of total RNA with Northern blotting and the reverse transcriptase polymerase chain reaction (RT-PCR). Large scale preparations containing over 10(9) cells (fresh, cultured with and without Con-A) originating from several donors were examined for the presence of POMC transcripts by analysis of poly(A)+ RNA on Northern blots and for the presence of alpha E, beta E, and gamma E by gel filtration over Sephadex G-75 and reverse phase HPLC, followed by assay of the fractions in four endorphin RIA systems with different specificities. On the Northern blots of total RNA, no POMC transcripts were detectable. In poly(A)+ RNA preparations, no full-length POMC mRNA was found, and it was estimated that the concentration of POMC mRNA, if present, was below approximately 0.005 transcript/cell in Con-A-stimulated cells and still lower in unstimulated cells. In accord with literature data, an 800- to 900-nucleotide POMC transcript was detected in cultured PBMC, and the levels of this transcript were stimulated by Con-A. In all samples analyzed with RT-PCR, a transcript spanning most of exons 2 and 3 was detectable only on Southern blots of the RT-PCR product, but not on agarose gels stained with ethidium bromide. Chromatographic analysis of endorphin immunoreactivities in cell extracts revealed no qualitative differences between the immunoreactive profiles of fresh PBMC or PBMC cultured with or without Con-A.(ABSTRACT TRUNCATED AT 400 WORDS)
1993
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1210/endo.133.5.8404638" target="_blank" rel="noreferrer">10.1210/endo.133.5.8404638</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
1993
Backlog
Base Sequence
beta-Endorphin/analysis
Biomarkers of Pain
Biomarkers Reference List
Blotting
Burbach JP
Cathepsin D/metabolism
Chromatography
de Wied D
DNA Probes
Endocrinology
Gel
High Pressure Liquid
Humans
Journal Article
Leukocytes
Lymphocytes/metabolism
Messenger/blood
Metzelaar MJ
Molecular Sequence Data
Mononuclear/metabolism
Northern
Peptide Fragments/analysis
Peptide Mapping
Polymerase Chain Reaction
Pro-Opiomelanocortin/genetics
RNA
Southern
van der Kleij AA
van Woudenberg AD
Wiegant VM