1
40
8
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1007/bf02828121" target="_blank" rel="noreferrer">http://doi.org/10.1007/bf02828121</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Leigh's syndrome
Publisher
An entity responsible for making the resource available
Indian Journal Of Pediatrics
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
Subject
The topic of the resource
Female; Humans; Disease Progression; Autopsy; Severity of Illness Index; Risk Assessment; Biopsy; Magnetic Resonance Imaging; Fatal Outcome; infant; Q3 Literature Search; Needle; Basal Ganglia/pathology; Immunohistochemistry; Leigh Disease/diagnosis/physiopathology/therapy
Creator
An entity primarily responsible for making the resource
Mannan AA; Sharma MC; Shrivastava P; Ralte AM; Gupta V; Behari M; Sarkar C
Description
An account of the resource
A 15-month-old female child presented with sudden onset cough and hyperventilation along with evidence of metabolic acidosis. She had past history of recurrent vomiting, episodes of abnormal posturing, difficulty in deglutition and regression of milestones since 12 months of age. CT scan of the brain revealed hypodense lesions in bilateral basal ganglia and on MRI there were T2 hyperintensities in bilateral lentiform nuclei, caudate nuclei, thalamus, red nuclei and dentate nuclei. Biochemical examination revealed persistently elevated serum lactate levels with high lactate/pyruvate ratio. Resuscitative measures were of no avail and the child succumbed to the illness on the second day of admission. Neuropathological examination at autopsy demonstrated marked spongiosis, focal necrosis, endothelial proliferation, reactive astrogliosis and extensive demyelination involving bilateral basal ganglia, midbrain and spinal cord which were typical of Leigh's sub acute necrotizing encephalomyelopathy.
2004
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/bf02828121" target="_blank" rel="noreferrer">10.1007/bf02828121</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
2004
Autopsy
Backlog
Basal Ganglia/pathology
Behari M
Biopsy
Disease Progression
Fatal Outcome
Female
Gupta V
Humans
Immunohistochemistry
Indian Journal Of Pediatrics
Infant
Journal Article
Leigh Disease/diagnosis/physiopathology/therapy
Magnetic Resonance Imaging
Mannan AA
Needle
Q3 Scoping Review Results
Ralte AM
Risk Assessment
Sarkar C
Severity Of Illness Index
Sharma MC
Shrivastava P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1016/s0165-5728(02)00049-8" target="_blank" rel="noreferrer">http://doi.org/10.1016/s0165-5728(02)00049-8</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Immunohistochemical localization of endomorphin-1 and endomorphin-2 in immune cells and spinal cord in a model of inflammatory pain
Publisher
An entity responsible for making the resource available
Journal Of Neuroimmunology
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
Subject
The topic of the resource
Male; Animals; Rats; Biomarkers of Pain; Immunohistochemistry; Freund's Adjuvant; Hindlimb; Wistar; Lymph Nodes/cytology; Lymphocytes/chemistry; Macrophages/chemistry; Monocytes/chemistry; Oligopeptides/analysis; Pain/chemically induced/immunology; Posterior Horn Cells/chemistry; Skin/chemistry/immunology/innervation
Creator
An entity primarily responsible for making the resource
Mousa SA; Machelska H; Schafer M; Stein C
Description
An account of the resource
Recently, two novel highly selective mu-opioid receptor (MOR) agonists, endomorphin-1 and endomorphin-2, have been isolated from bovine as well as human brains and were proposed to be the endogenous ligand for MOR. Later, endomorphin-1 and endomorphin-2 have been detected in the immune system of rats and humans using radioimmunoassay in combination with reverse-high-phase-liquid chromatography. In the present study, we analyzed the expression of endomorphin-1, endomorphin-2 and MOR by immunohistochemistry in a model of Freund's complete adjuvant (FCA)-induced painful inflammation. While MOR was upregulated on peripheral and central nerve terminals, inflammation did not alter endomorphin-2 expression in nerve fibers either in the dorsal horn of the spinal cord or in subcutaneous tissue. Endomorphin-1 and endomorphin-2 were expressed in immune cells (macrophage/monocytes) in the medullary region of the popliteal lymph nodes. The proportion of immunocytes (macrophage/monocytes, lymphocytes) containing endomorphin-1 and endomorphin-2 was increased in inflamed lymph nodes and subcutaneous paw tissue of animals with local inflammatory pain. Taken together, the upregulation of MOR and of its endogenous ligands endomorphin-1 and endomorphin-2 in immunocytes suggests an involvement of these opioid peptides in the peripheral control of inflammatory pain.
2002
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0165-5728(02)00049-8" target="_blank" rel="noreferrer">10.1016/s0165-5728(02)00049-8</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
2002
Animals
Backlog
Biomarkers of Pain
Freund's Adjuvant
Hindlimb
Immunohistochemistry
Journal Article
Journal Of Neuroimmunology
Lymph Nodes/cytology
Lymphocytes/chemistry
Machelska H
Macrophages/chemistry
Male
Monocytes/chemistry
Mousa SA
Oligopeptides/analysis
Pain/chemically induced/immunology
Posterior Horn Cells/chemistry
Rats
Schafer M
Skin/chemistry/immunology/innervation
Stein C
Wistar
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1097/00000542-200301000-00030" target="_blank" rel="noreferrer">http://doi.org/10.1097/00000542-200301000-00030</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Modulation of peripheral endogenous opioid analgesia by central afferent blockade
Publisher
An entity responsible for making the resource available
Anesthesiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
Subject
The topic of the resource
Male; Analgesics; Animals; Rats; Injections; Immunohistochemistry; Spinal; Enkephalin; beta-Endorphin/metabolism; Wistar; Pain Threshold/drug effects; Neurons; Afferent/drug effects; Central Nervous System/drug effects; Endorphins/metabolism/physiology; Flow Cytometry; Foot/pathology; Inflammation/pathology; Methionine/metabolism; Morphine/administration & dosage/pharmacology; Opioid/administration & dosage/pharmacology; Peripheral Nerves/drug effects; Psychomotor Performance/drug effects
Creator
An entity primarily responsible for making the resource
Schmitt TK; Mousa SA; Brack A; Schmidt DK; Rittner HL; Welte M; Schafer M; Stein C
Description
An account of the resource
BACKGROUND: Peripheral tissue injury causes a migration of opioid peptide-containing immune cells to the inflamed site. The subsequent release and action of these peptides on opioid receptors localized on peripheral sensory nerve terminals causes endogenous analgesia. The spinal application of opioid drugs blocks the transmission of nociceptive information from peripheral injury. This study investigates the influence of exogenous spinal opioid analgesia on peripheral endogenous opioid analgesia. METHODS: Six and forty-eight hours after initiation of continuous intrathecal morphine infusion and administration of Freund's complete adjuvant into the hind paw of rats, antinociceptive and antiinflammatory effects were measured by paw pressure threshold, paw volume, and paw temperature, respectively. Inflammation and quantity of opioid-containing cells were evaluated by immunocytochemistry and flow cytometry. Cold water swim stress-induced endogenous analgesia was examined 24 h after discontinuation of intrathecal morphine administration. RESULTS: Intrathecal morphine (10 micro g/h) resulted in a significant and stable increase of paw pressure threshold ( P 0.05). At 48 but not at 6 h after Freund's complete adjuvant, the number of beta-endorphin-containing cells and cold water swim-induced antinociception were significantly reduced in intrathecal morphine-treated rats compared with those treated with intrathecal vehicle ( P< 0.05). CONCLUSIONS: These findings suggest an interplay of central and peripheral mechanisms of pain control. An effective central inhibition of pain apparently signals a reduced need for recruitment of opioid-containing immune cells to injured sites.
2003
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1097/00000542-200301000-00030" target="_blank" rel="noreferrer">10.1097/00000542-200301000-00030</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
2003
Afferent/drug effects
Analgesics
Anesthesiology
Animals
Backlog
beta-Endorphin/metabolism
Brack A
Central Nervous System/drug effects
Endorphins/metabolism/physiology
Enkephalin
Flow Cytometry
Foot/pathology
Immunohistochemistry
Inflammation/pathology
Injections
Journal Article
Male
Methionine/metabolism
Morphine/administration & dosage/pharmacology
Mousa SA
Neurons
Opioid/administration & dosage/pharmacology
Pain Threshold/drug effects
Peripheral Nerves/drug effects
Psychomotor Performance/drug effects
Rats
Rittner HL
Schafer M
Schmidt DK
Schmitt TK
Spinal
Stein C
Welte M
Wistar
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1097/01.bpb.0000049567.52224.fa" target="_blank" rel="noreferrer">http://doi.org/10.1097/01.bpb.0000049567.52224.fa</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Modern approach to children with osteogenesis imperfecta
Publisher
An entity responsible for making the resource available
Journal Of Pediatric Orthopaedics. Part B / European Paediatric Orthopaedic Society, Pediatric Orthopaedic Society Of North America
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
Subject
The topic of the resource
Child; Female; Humans; Male; Prognosis; Treatment Outcome; Severity of Illness Index; Risk Assessment; Biopsy; Combined Modality Therapy; Preschool; infant; Needle; Clodronate; Diphosphonates/therapeutic use; Immunohistochemistry; Bone Density/physiology; Physical Therapy Modalities; Orthopedic Fixation Devices; Orthopedic Procedures/instrumentation/methods; Osteogenesis Imperfecta/pathology/therapy
Creator
An entity primarily responsible for making the resource
Zeitlin L; Fassier F; Glorieux FH
Description
An account of the resource
Osteogenesis Imperfecta (OI) is characterized by bone fragility. At least seven discrete types have been described ranging from mild disease to a lethal form. In a large number of cases, mutations in one of the two genes encoding type I collagen have been found. In forms recently described (types V, VI, VII), such mutations have been excluded. In two other forms, (Bruck, and osteoporosis - pseudoglioma syndromes) defects in other proteins have been characterized. In OI, bone fragility stems from: decreased bone mass, disturbed organization of bone tissue, and altered bone geometry (size and shape). Histologic studies have shown that increased bone turnover is the rule in OI bone. This justifies using bisphosphonates in order to reduce osteoclast mediated bone resorption. Initial results are encouraging. Cyclical intravenous pamidronate administration reduces bone pain and fracture incidence, and increases bone density and level of ambulation, with minimal side effects. Effects on bone include increase in size of vertebral bodies and thickening of cortical bone. These results allow for more efficacious corrective surgery using intramedullary rodding of the long bones and paravertegral instrumentation. Specific occupational and physiotherapy programs are integral parts of the treatment protocol. This multidisciplinary approach will prevail until strategies aiming at the correction of the basic defect(s) will have come to fruition.
2003
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1097/01.bpb.0000049567.52224.fa" target="_blank" rel="noreferrer">10.1097/01.bpb.0000049567.52224.fa</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
2003
Backlog
Biopsy
Bone Density/physiology
Child
Clodronate
Combined Modality Therapy
Diphosphonates/therapeutic use
Fassier F
Female
Glorieux FH
Humans
Immunohistochemistry
Infant
Journal Article
Journal Of Pediatric Orthopaedics. Part B / European Paediatric Orthopaedic Society, Pediatric Orthopaedic Society Of North America
Male
Needle
Orthopedic Fixation Devices
Orthopedic Procedures/instrumentation/methods
Osteogenesis Imperfecta/pathology/therapy
Physical Therapy Modalities
Preschool
Prognosis
Risk Assessment
Severity Of Illness Index
Treatment Outcome
Zeitlin L
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1210/en.2003-1287" target="_blank" rel="noreferrer">http://doi.org/10.1210/en.2003-1287</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Subcellular pathways of beta-endorphin synthesis, processing, and release from immunocytes in inflammatory pain
Publisher
An entity responsible for making the resource available
Endocrinology
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
Subject
The topic of the resource
Male; Animals; Rats; Biomarkers of Pain; Microscopy; Immunohistochemistry; Wistar; beta-Endorphin/biosynthesis; Carboxypeptidase H/metabolism; Extremities; Immunoelectron; Inflammation/immunology/metabolism; Leukocytes/drug effects/metabolism/ultrastructure; Norepinephrine/pharmacology; Pain/immunology/metabolism; Pro-Opiomelanocortin/metabolism; Proprotein Convertase 1/metabolism; Proprotein Convertase 2/metabolism; Secretory Vesicles/metabolism/ultrastructure; Sympathomimetics/pharmacology
Creator
An entity primarily responsible for making the resource
Mousa SA; Shakibaei M; Sitte N; Schafer M; Stein C
Description
An account of the resource
The opioid peptide beta-endorphin (END) as well as mRNA for its precursor proopiomelanocortin (POMC) are found not only in the pituitary gland, but also within various types of immune cells infiltrating inflamed sc tissue. During stressful stimuli END is released and interacts with peripheral opioid receptors to inhibit pain. However, the subcellular pathways of POMC processing and END release have not yet been delineated in inflammatory cells. The aim of the present study was to examine the presence of POMC, carboxypeptidase E, the prohormone convertases 1 (PC1), and 2 (PC2), PC2-binding protein 7B2, and the release of END from inflammatory cells in rats. Using immunohistochemistry we detected END and POMC alone or colocalized with PC1, PC2, carboxypeptidase E, and 7B2 in macrophages/monocytes, granulocytes, and lymphocytes of the blood and within inflamed sc paw tissue. Immunoelectron microscopy revealed that END is localized within secretory granules packed in membranous structures in macrophages, monocytes, granulocytes, and lymphocytes. Finally, END is released by noradrenaline from immune cells in vitro. Taken together, our results indicate that immune cells express the entire machinery required for POMC processing into functionally active peptides such as END and are able to release these peptides from secretory granules.
2004
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1210/en.2003-1287" target="_blank" rel="noreferrer">10.1210/en.2003-1287</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
2004
Animals
Backlog
beta-Endorphin/biosynthesis
Biomarkers of Pain
Carboxypeptidase H/metabolism
Endocrinology
Extremities
Immunoelectron
Immunohistochemistry
Inflammation/immunology/metabolism
Journal Article
Leukocytes/drug effects/metabolism/ultrastructure
Male
Microscopy
Mousa SA
Norepinephrine/pharmacology
Pain/immunology/metabolism
Pro-Opiomelanocortin/metabolism
Proprotein Convertase 1/metabolism
Proprotein Convertase 2/metabolism
Rats
Schafer M
Secretory Vesicles/metabolism/ultrastructure
Shakibaei M
Sitte N
Stein C
Sympathomimetics/pharmacology
Wistar
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1016/0140-6736(93)91471-w" target="_blank" rel="noreferrer">http://doi.org/10.1016/0140-6736(93)91471-w</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Local analgesic effect of endogenous opioid peptides
Publisher
An entity responsible for making the resource available
Lancet
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
Subject
The topic of the resource
Humans; Pain; Adult; Aged; Middle Aged; Double-Blind Method; Biomarkers of Pain; Injections; Intravenous; Receptors; Opioid/drug effects; Immunohistochemistry; Enkephalin; Arthroscopy; beta-Endorphin/analysis/physiology; Dynorphins/analysis/physiology; Endorphins/analysis/physiology; Intra-Articular; Knee Joint/surgery; Methionine/analysis/physiology; Naloxone/administration & dosage/pharmacology; Postoperative/etiology; Synovial Membrane/chemistry; Synovitis/metabolism
Creator
An entity primarily responsible for making the resource
Stein C; Hassan AH; Lehrberger K; Giefing J; Yassouridis A
Description
An account of the resource
Opioids produce analgesia by interacting with local opioid receptors in peripheral inflamed tissue. This study investigated whether endogenous ligands of these receptors are present in synovia and whether such opioid peptides can inhibit pain by activation of intra-articular opioid receptors. Samples of synovium from 8 patients undergoing arthroscopic knee surgery were examined by immunohistochemistry for the presence of beta-endorphin, met-enkephalin, and dynorphin. All tissue samples showed synovitis. Inflammatory cells stained strongly for beta-endorphin and met-enkephalin but not for dynorphin. To find out whether blockade of intra-articular opioid receptors affected pain, we randomly assigned 22 patients undergoing arthroscopic knee surgery to receive naloxone (0.04 mg) intra-articularly (n = 10) or intravenously (n = 12); each patient received a placebo injection into the other site. Postoperative pain was assessed by visual analogue scale, a numerical rating scale, the McGill pain questionnaire, and supplementary analgesic consumption during the next 24 h. All pain scores were higher in the intra-articular naloxone group than in the intravenous naloxone group. The differences were significant (p < 0.05) during the first 4 h. Supplementary analgesic consumption was significantly higher in the intra-articular group (52.5 [14.0] vs 15.6 [8.0] mg diclofenac, p < 0.05). Opioid peptides are present in inflamed synovial tissue and can inhibit pain after knee surgery through an action specific to intra-articular opioid receptors. These findings expand the gate control theory of pain and suggest new approaches such as the development of peripherally acting opioid analgesics without central side-effects.
1993
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0140-6736(93)91471-w" target="_blank" rel="noreferrer">10.1016/0140-6736(93)91471-w</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
1993
Adult
Aged
Arthroscopy
Backlog
beta-Endorphin/analysis/physiology
Biomarkers of Pain
Double-Blind Method
Dynorphins/analysis/physiology
Endorphins/analysis/physiology
Enkephalin
Giefing J
Hassan AH
Humans
Immunohistochemistry
Injections
Intra-Articular
Intravenous
Journal Article
Knee Joint/surgery
Lancet
Lehrberger K
Methionine/analysis/physiology
Middle Aged
Naloxone/administration & dosage/pharmacology
Opioid/drug effects
Pain
Postoperative/etiology
Receptors
Stein C
Synovial Membrane/chemistry
Synovitis/metabolism
Yassouridis A
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1038/nm0897-831" target="_blank" rel="noreferrer">http://doi.org/10.1038/nm0897-831</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Pain killers of the immune system
Publisher
An entity responsible for making the resource available
Nature Medicine
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
Subject
The topic of the resource
Animals; Rats; Biomarkers of Pain; RNA; Immunohistochemistry; Radioimmunoassay; beta-Endorphin/biosynthesis/physiology; Inflammation/pathology/physiopathology; Messenger/genetics; Pain/pathology/physiopathology; Pro-Opiomelanocortin/genetics; T-Lymphocytes/metabolism
Creator
An entity primarily responsible for making the resource
Sharp B; Yaksh T
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1038/nm0897-831" target="_blank" rel="noreferrer">10.1038/nm0897-831</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
Description
An account of the resource
1997
1997
Animals
Backlog
beta-Endorphin/biosynthesis/physiology
Biomarkers of Pain
Immunohistochemistry
Inflammation/pathology/physiopathology
Journal Article
Messenger/genetics
Nature Medicine
Pain/pathology/physiopathology
Pro-Opiomelanocortin/genetics
Radioimmunoassay
Rats
RNA
Sharp B
T-Lymphocytes/metabolism
Yaksh T
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1097/00000542-200108000-00036" target="_blank" rel="noreferrer">http://doi.org/10.1097/00000542-200108000-00036</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Opioid peptide-expressing leukocytes: identification, recruitment, and simultaneously increasing inhibition of inflammatory pain
Publisher
An entity responsible for making the resource available
Anesthesiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
Subject
The topic of the resource
Male; Pain Measurement; Analgesia; Animals; Rats; beta-Endorphin/blood; Biomarkers of Pain; Pain/physiopathology; Immunohistochemistry; Radioimmunoassay; Biomarkers Reference List; Wistar; Antibodies; Antigens; CD45/isolation & purification; Fluorescent Dyes; Hematopoietic Stem Cells/immunology; Immunomagnetic Separation; Inflammation/chemically induced/metabolism/pathology; Leukocytes/metabolism; Lymphocytes/immunology; Monoclonal/pharmacology; Opioid Peptides/biosynthesis
Creator
An entity primarily responsible for making the resource
Rittner HL; Brack A; Machelska H; Mousa SA; Bauer M; Schafer M; Stein C
Description
An account of the resource
BACKGROUND: Inflammatory pain can be effectively controlled by an interaction of opioid receptors on peripheral sensory nerve terminals with opioid peptides released from immune cells upon stressful stimulation. To define the source of opioid peptide production, we sought to identify and quantify populations of opioid-containing cells during the course of Freund's complete adjuvant-induced hind paw inflammation in the rat. In parallel, we examined the development of stress-induced local analgesia in the paw. METHODS: At 2, 6, and 96 h after Freund's complete adjuvant inoculation, cells were characterized by flow cytometry using a monoclonal pan-opioid antibody (3E7) and antibodies against cell surface antigens and by immunohistochemistry using a polyclonal antibody to beta-endorphin. After magnetic cell sorting, the beta-endorphin content was quantified by radioimmunoassay. Pain responses before and after cold water swim stress were evaluated by paw pressure thresholds. RESULTS: In early inflammation, 66% of opioid peptide-producing (3E7+) leukocytes were HIS48+ granulocytes. In contrast, at later stages (96 h), the majority of 3E7+ immune cells were ED1+ monocytes or macrophages (73%). During the 4 days after Freund's complete adjuvant inoculation, the number of 3E7+ cells increased 5.6-fold (P < 0.001, Kruskal-Wallis test) and the beta-endorphin content in the paw multiplied 3.9-fold (P < 0.05, Kruskal-Wallis test). In parallel, cold water swim stress-induced analgesia increased by 160% (P < 0.01, analysis of variance). CONCLUSIONS: The degree of endogenous pain inhibition is proportional to the number of opioid peptide-producing cells, and distinct leukocyte lineages contribute to this function at different stages of inflammation. These mechanisms may be important for understanding pain in immunosuppressed states such as cancer, diabetes, or AIDS and for the design of novel therapeutic strategies in inflammatory diseases.
2001
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1097/00000542-200108000-00036" target="_blank" rel="noreferrer">10.1097/00000542-200108000-00036</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
2001
Analgesia
Anesthesiology
Animals
Antibodies
Antigens
Backlog
Bauer M
beta-Endorphin/blood
Biomarkers of Pain
Biomarkers Reference List
Brack A
CD45/isolation & purification
Fluorescent Dyes
Hematopoietic Stem Cells/immunology
Immunohistochemistry
Immunomagnetic Separation
Inflammation/chemically induced/metabolism/pathology
Journal Article
Leukocytes/metabolism
Lymphocytes/immunology
Machelska H
Male
Monoclonal/pharmacology
Mousa SA
Opioid Peptides/biosynthesis
Pain Measurement
Pain/physiopathology
Radioimmunoassay
Rats
Rittner HL
Schafer M
Stein C
Wistar