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Text
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<a href="http://doi.org/10.1016/s0165-5728(99)00216-7" target="_blank" rel="noreferrer">http://doi.org/10.1016/s0165-5728(99)00216-7</a>
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Title
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Novel opioid peptides endomorphin-1 and endomorphin-2 are present in mammalian immune tissues
Publisher
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Journal Of Neuroimmunology
Date
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2000
Subject
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Female; Humans; Male; Adult; Middle Aged; Animals; Rats; Non-U.S. Gov't; Research Support; Wistar; Radioimmunoassay/methods; Chromatography; High Pressure Liquid; Oligopeptides/analysis; Cross Reactions; Immune Sera/immunology; Immune System/chemistry; Spleen/chemistry; Thymus Gland/chemistry; Tissue Extracts/chemistry
Creator
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Jessop DS; Major GN; Coventry TL; Kaye SJ; Fulford AJ; Harbuz MS; De Bree FM
Description
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Endomorphin (EM)-1 and EM-2 are opioid tetrapeptides, reported within the central nervous system, which have very high specificity and affinity for the mu-opioid receptor. We have used newly developed and well-characterised radioimmunoassays (RIAs) in combination with reversed-phase high-performance liquid chromatography (HPLC) to detect EM-1 and EM-2 immunoreactivity (ir) in rat immune tissues. Endomorphins were detectable in extracts of rat spleen (total EM-1-ir/spleen: 440+/-73 pg, mean+/-SEM, a=group of eight rats; EM-2-ir: 150+/-12 pg) and thymus (EM-1-ir: 152+/-18 pg, mean+/-SEM n=8; EM-2-ir: 156+/-28 pg). EM-2-ir was detectable in extracts of human spleen (338+/-196 pg/g tissue, n=3). Multiple peaks of EM-1-ir and EM-2-ir were observed in rat spleen and thymus extracts, and multiple peaks of EM-2-ir were observed in extracts of human spleen, following reversed-phase HPLC and RIAs. This is the first report of endomorphin immunoreactivity in tissues of the rat and human immune systems.
2000
Identifier
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<a href="http://doi.org/10.1016/s0165-5728(99)00216-7" target="_blank" rel="noreferrer">10.1016/s0165-5728(99)00216-7</a>
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Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
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Journal Article
2000
Adult
Animals
Backlog
Chromatography
Coventry TL
Cross Reactions
De Bree FM
Female
Fulford AJ
Harbuz MS
High Pressure Liquid
Humans
Immune Sera/immunology
Immune System/chemistry
Jessop DS
Journal Article
Journal Of Neuroimmunology
Kaye SJ
Major GN
Male
Middle Aged
Non-U.S. Gov't
Oligopeptides/analysis
Radioimmunoassay/methods
Rats
Research Support
Spleen/chemistry
Thymus Gland/chemistry
Tissue Extracts/chemistry
Wistar