1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Citation List Month Backlog URL Address <a href="http://doi.org/10.1097/00000542-200401000-00024" target="_blank" rel="noreferrer">http://doi.org/10.1097/00000542-200401000-00024</a> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Mobilization of opioid-containing polymorphonuclear cells by hematopoietic growth factors and influence on inflammatory pain Publisher An entity responsible for making the resource available Anesthesiology Date A point or period of time associated with an event in the lifecycle of the resource 2004 Subject The topic of the resource Male; Animals; Rats; Biomarkers of Pain; Inflammation/complications; RNA; Pain Measurement/drug effects; Radioimmunoassay; Pain/drug therapy/physiopathology; Wistar; Neutrophils/metabolism; Messenger/biosynthesis; DNA Primers; Reverse Transcriptase Polymerase Chain Reaction; Flow Cytometry; Cell Adhesion Molecules/pharmacology; Cell Separation; Chemokines/biosynthesis; Chemotaxis; Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology; Hindlimb/physiology; Leukocyte/drug effects; Light; Narcotics/metabolism/pharmacology; Stem Cell Factor/pharmacology Creator An entity primarily responsible for making the resource Brack A; Rittner HL; Machelska H; Beschmann K; Sitte N; Schafer M; Stein C Description An account of the resource BACKGROUND: Leukocytes can control inflammatory pain by secretion of opioid peptides, stimulated by cold-water swimming or local injection of corticotropin-releasing factor, and subsequent activation of opioid receptors on peripheral sensory neurons. This study investigated whether mobilization of polymorphonuclear cells (PMN) by granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) enhances immigration of opioid-containing PMN and peripheral opioid analgesia in rats with Freund complete adjuvant-induced hind paw inflammation. METHODS: In circulating PMN of rats treated with G-CSF+SCF and sham-treated rats, opioid peptide content was measured by radioimmunoassay. Expression of adhesion molecules (CD62L, CD49d, CD18), in vitro migration in the Boyden chamber, and infiltrating leukocytes were analyzed by flow cytometry. Chemokine messenger RNA transcription was quantified by LightCycler polymerase chain reaction. Paw pressure threshold was measured at baseline, after cold-water swimming, and after injection of corticotropin-releasing factor. RESULTS: G-CSF+SCF treatment increased circulating PMN (11-fold, P 0.05). CONCLUSIONS: G-CSF+SCF mobilized circulating opioid-containing PMN but had a minor influence on cell migration and peripheral analgesia, probably because of the low expression of chemokines in the inflamed paw and one of the decreased beta-endorphin content in PMN. 2004 Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1097/00000542-200401000-00024" target="_blank" rel="noreferrer">10.1097/00000542-200401000-00024</a> Rights Information about rights held in and over the resource Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s). Type The nature or genre of the resource Journal Article 2004 Anesthesiology Animals Backlog Beschmann K Biomarkers of Pain Brack A Cell Adhesion Molecules/pharmacology Cell Separation Chemokines/biosynthesis Chemotaxis DNA Primers Flow Cytometry Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology Hindlimb/physiology Inflammation/complications Journal Article Leukocyte/drug effects Light Machelska H Male Messenger/biosynthesis Narcotics/metabolism/pharmacology Neutrophils/metabolism Pain Measurement/drug effects Pain/drug therapy/physiopathology Radioimmunoassay Rats Reverse Transcriptase Polymerase Chain Reaction Rittner HL RNA Schafer M Sitte N Stein C Stem Cell Factor/pharmacology Wistar