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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
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URL Address
<a href="http://doi.org/10.1016/s0304-3959(01)00322-0" target="_blank" rel="noreferrer">http://doi.org/10.1016/s0304-3959(01)00322-0</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Methionine-enkephalin-and Dynorphin A-release from immune cells and control of inflammatory pain
Publisher
An entity responsible for making the resource available
Pain
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
Subject
The topic of the resource
Humans; Male; Animals; Rats; Biomarkers of Pain; Dose-Response Relationship; Drug; Enkephalin; Biomarkers Reference List; Wistar; Antibodies/pharmacology; Corticotropin-Releasing Hormone/antagonists & inhibitors/pharmacology/therapeutic use; Dynorphins/immunology/secretion; Inflammation/drug therapy/immunology/metabolism; Interleukin-2/pharmacology; Lymphocytes/drug effects/immunology/secretion; Methionine/immunology/metabolism; Pain/drug therapy/immunology/metabolism
Creator
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Cabot PJ; Carter L; Schafer M; Stein C
Description
An account of the resource
We have previously shown that beta-endorphin (END) is contained and released from memory-type T-cells within inflamed tissue and that it is capable to control pain (J Clin Invest 100(1) (1997) 142). Methionine-enkephalin (MET) and Dynorphin-A (DYN) are endogenous opioids with preference for delta- and kappa-opioid receptors, respectively. Both MET and DYN are produced and contained within immune cells. The goal of this study was to determine the release characteristics of MET and DYN in a rat model of localized hindpaw inflammation and to examine the antinociceptive role of MET and DYN in a Freund's adjuvant induced model of inflammatory pain. We found that corticotropin-releasing factor (CRF) can stimulate the release of both MET and DYN from lymphocytes. This release is dose-dependent and reversible by the selective CRF antagonist alpha-helical-CRF. Furthermore, CRF (1.5 ng) produces analgesia when injected into the inflamed paw, which is reversible by direct co-administration of antibodies to MET. Lymphocyte content of MET was 7.0+/-1.4 ng/million cells, whilst DYN content was ~30-fold lower. Both END and DYN, but not MET, were released by IL-1. Consistently, IL-1 produced peripheral analgesic effects which were not reversed by antibodies to MET. These results indicate that both MET and DYN play a role in peripheral analgesia but have different characteristics of release. These studies further support a role of the immune system in the control of inflammatory pain. This may be particularly important in patients suffering from compromised immune systems as with cancer and AIDS.
2001
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0304-3959(01)00322-0" target="_blank" rel="noreferrer">10.1016/s0304-3959(01)00322-0</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
2001
Animals
Antibodies/pharmacology
Backlog
Biomarkers of Pain
Biomarkers Reference List
Cabot PJ
Carter L
Corticotropin-Releasing Hormone/antagonists & inhibitors/pharmacology/therapeutic use
Dose-Response Relationship
Drug
Dynorphins/immunology/secretion
Enkephalin
Humans
Inflammation/drug therapy/immunology/metabolism
Interleukin-2/pharmacology
Journal Article
Lymphocytes/drug effects/immunology/secretion
Male
Methionine/immunology/metabolism
Pain
Pain/drug therapy/immunology/metabolism
Rats
Schafer M
Stein C
Wistar
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Citation List Month
Backlog
URL Address
<a href="http://doi.org/10.1172/jci119506" target="_blank" rel="noreferrer">http://doi.org/10.1172/jci119506</a>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Immune cell-derived beta-endorphin. Production, release, and control of inflammatory pain in rats
Publisher
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The Journal Of Clinical Investigation
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
Subject
The topic of the resource
Humans; Male; Time Factors; Analysis of Variance; Animals; Regression Analysis; Rats; Biomarkers of Pain; RNA; Genetic; Biomarkers Reference List; Inflammation/physiopathology; Freund's Adjuvant; Hindlimb; Pain/immunology/physiopathology; Corticotropin-Releasing Hormone/pharmacology; Wistar; Messenger/biosynthesis; beta-Endorphin/biosynthesis; Interleukin-1/pharmacology; Lymph Nodes/metabolism; Pro-Opiomelanocortin/biosynthesis; T-Lymphocytes/drug effects/immunology/metabolism; Transcription
Creator
An entity primarily responsible for making the resource
Cabot PJ; Carter L; Gaiddon C; Zhang Q; Schafer M; Loeffler JP; Stein C
Description
An account of the resource
Localized inflammation of a rat's hindpaw elicits an accumulation of beta-endorphin-(END) containing immune cells. We investigated the production, release, and antinociceptive effects of lymphocyte-derived END in relation to cell trafficking. In normal animals, END and proopiomelanocortin mRNA were less abundant in circulating lymphocytes than in those residing in lymph nodes (LN), suggesting that a finite cell population produces END and homes to LN. Inflammation increased proopiomelanocortin mRNA in cells from noninflamed and inflamed LN. However, END content was increased only in inflamed paw tissue and noninflamed LN-immune cells. Accordingly, corticotropin-releasing factor and IL-1beta released significantly more END from noninflamed than from inflamed LN-immune cells. This secretion was receptor specific, calcium dependent, and mimicked by potassium, consistent with vesicular release. Finally, both agents, injected into the inflamed paw, induced analgesia which was blocked by the co-administration of antiserum against END. Together, these findings suggest that END-producing lymphocytes home to inflamed tissue where they secrete END to reduce pain. Afterwards they migrate to the regional LN, depleted of the peptide. Consistent with this notion, immunofluorescence studies of cell suspensions revealed that END is contained predominantly within memory-type T cells. Thus, the immune system is important for the control of inflammatory pain. This has implications for the understanding of pain in immunosuppressed conditions like cancer or AIDS.
1997
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1172/jci119506" target="_blank" rel="noreferrer">10.1172/jci119506</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. PedPalASCNET does not hold any rights over the resource listed here. All rights are retained by the journal listed under publisher and/or the creator(s).
Type
The nature or genre of the resource
Journal Article
1997
Analysis of Variance
Animals
Backlog
beta-Endorphin/biosynthesis
Biomarkers of Pain
Biomarkers Reference List
Cabot PJ
Carter L
Corticotropin-Releasing Hormone/pharmacology
Freund's Adjuvant
Gaiddon C
Genetic
Hindlimb
Humans
Inflammation/physiopathology
Interleukin-1/pharmacology
Journal Article
Loeffler JP
Lymph Nodes/metabolism
Male
Messenger/biosynthesis
Pain/immunology/physiopathology
Pro-Opiomelanocortin/biosynthesis
Rats
Regression Analysis
RNA
Schafer M
Stein C
T-Lymphocytes/drug effects/immunology/metabolism
The Journal Of Clinical Investigation
Time Factors
transcription
Wistar
Zhang Q